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anti becn1 a7353 pab  (ABclonal Biotechnology)


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    Structured Review

    ABclonal Biotechnology anti becn1 a7353 pab
    Anti Becn1 A7353 Pab, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/beclin-1+(a7353)/%5BKO+Validated%5D+Beclin+1+Rabbit+pAb/pmc13386971-202-8-27
    Average 95 stars, based on 77 article reviews
    anti becn1 a7353 pab - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Membrane:

    Article Title: ADAMTS13 ameliorates diabetic nephropathy by Nrf2/GPX4/eNOS signaling pathway
    Article Snippet: Nuclear and cytoplasmic proteins were extracted using a commercial kit (P0027, Beyotime, Shanghai, China). .. Equal amounts of protein were loaded on 8–12% sodium dodecyl sulfate-polyacrylamide gels, transferred to polyvinylidene fluoride membrane (PVDF) (Thermo Fisher Scientific, Waltham, MA), and blocked with 5% skim milk powder for 2 h. Membranes were incubated overnight at 4 °C with the following primary antibodies: Beclin-1 (A7353, ABclonal, Wuhan, China); LC3II/I (#4108, 14/16 kDa, CST, Danvers, MA); Nrf2 (ab31163, Abcam, Cambridge, UK); SOD1 (A12537, ABclonal, Wuhan, China); SOD2 (A21805, ABclonal, Wuhan, China); GPX4 (A1933, ABclonal, Wuhan, China); eNOS (#32027, CST, Danvers, MA); p-eNOS (Ser 1177) (AF5812, Beyotime, Shanghai, China); β-actin (AF5002, Beyotime, Shanghai, China); GAPDH (AC001, ABclonal, Wuhan, China); Histone H3 (A2348, ABclonal, Wuhan, China). .. Thereafter, membranes were washed three times with TBST buffer; they were probed with horseradish-peroxidase-conjugated secondary antibodies ((rabbit, ab6721, Abcam, Cambridge, UK); (mouse, ab6789, Abcam, Cambridge, UK)) for 1 h at room temperature.

    Article Title: Naringin alleviates autoimmune hepatitis in mice via the gut-liver Axis through modulation of microbiota, metabolites, and immune responses.
    Article Snippet: Autoimmune hepatitis (AIH) is an immune-mediated liver disease that could be impacted by gut microbiota dysbiosis.. Naringin, a flavonoid derived from citrus fruits, has been reported to modulate gut microbial composition and alleviate liver diseases, but its role in AIH remains incompletely understood.. In this study, we used multi-omics analysis and fecal microbiota transplantation (FMT) to examine the protective benefits of naringin in a ConA-induced AIH mouse model. To strengthen mechanistic conclusions, histopathological scoring, liver function indices, and immune cell profiling were determined.

    Incubation:

    Article Title: ADAMTS13 ameliorates diabetic nephropathy by Nrf2/GPX4/eNOS signaling pathway
    Article Snippet: Nuclear and cytoplasmic proteins were extracted using a commercial kit (P0027, Beyotime, Shanghai, China). .. Equal amounts of protein were loaded on 8–12% sodium dodecyl sulfate-polyacrylamide gels, transferred to polyvinylidene fluoride membrane (PVDF) (Thermo Fisher Scientific, Waltham, MA), and blocked with 5% skim milk powder for 2 h. Membranes were incubated overnight at 4 °C with the following primary antibodies: Beclin-1 (A7353, ABclonal, Wuhan, China); LC3II/I (#4108, 14/16 kDa, CST, Danvers, MA); Nrf2 (ab31163, Abcam, Cambridge, UK); SOD1 (A12537, ABclonal, Wuhan, China); SOD2 (A21805, ABclonal, Wuhan, China); GPX4 (A1933, ABclonal, Wuhan, China); eNOS (#32027, CST, Danvers, MA); p-eNOS (Ser 1177) (AF5812, Beyotime, Shanghai, China); β-actin (AF5002, Beyotime, Shanghai, China); GAPDH (AC001, ABclonal, Wuhan, China); Histone H3 (A2348, ABclonal, Wuhan, China). .. Thereafter, membranes were washed three times with TBST buffer; they were probed with horseradish-peroxidase-conjugated secondary antibodies ((rabbit, ab6721, Abcam, Cambridge, UK); (mouse, ab6789, Abcam, Cambridge, UK)) for 1 h at room temperature.

    Article Title: The cGAS-STING pathway contributes to cisplatin-induced skeletal muscle atrophy through altered proteostasis and myogenic signaling.
    Article Snippet: .. After transfer to PVDF membranes, blots were blocked in 5% non-fat milk and incubated overnight at 4 °C with primary antibodies against FBXO32 (ABclonal, A3193, 1:1000), Beclin1 (ABclonal, A7353, 1:1000), MyoD (ABclonal, A23881, 1:1000), LC3-I/II (Servicebio, GBGB11124, 1:5000), and GAPDH (Servicebio, GB15002, 1:2000). .. Membranes were then incubated with HRP-conjugated secondary antibodies (Servicebio, Wuhan, China), and signals were detected by enhanced chemiluminescence.

    Article Title: Naringin alleviates autoimmune hepatitis in mice via the gut-liver Axis through modulation of microbiota, metabolites, and immune responses.
    Article Snippet: Autoimmune hepatitis (AIH) is an immune-mediated liver disease that could be impacted by gut microbiota dysbiosis.. Naringin, a flavonoid derived from citrus fruits, has been reported to modulate gut microbial composition and alleviate liver diseases, but its role in AIH remains incompletely understood.. In this study, we used multi-omics analysis and fecal microbiota transplantation (FMT) to examine the protective benefits of naringin in a ConA-induced AIH mouse model. To strengthen mechanistic conclusions, histopathological scoring, liver function indices, and immune cell profiling were determined.

    other:

    Article Title: Indole-alkaloid–rich fraction of Ervatamia coronaria leaf extract regresses breast cancer by inducing apoptotic cell death
    Article Snippet: Concentration of proteins in the whole cell lysate was measured by using Bradford reagent (Bio-Rad) by UV–VIS spectrophotometer (Multiscan Go from Thermo Scientific) at 595 nm [ ]. (Antibodies used with catalogue number: ATG7 D12B11, CASPASE 7 #9492 from Cell Signalling Technology, USA, PARP-1 cat no A0942, BECLIN-1 cat no A7353, Abclonal, USA, CLEAVED CASPASE 3 cat no 9664S, from CST USA, LC3B (KO validated) cat no A7198, β-ACTIN Catalogue no# BB-AB0024 from BioBharti LifeScience, India, HRP-Goat anti-rabbit secondary antibody was from BioBharti LifeScience, India, Catalogue no# BB-SAB01B.)

    Article Title: Cleavage of TOM1 by the SARS-CoV-2 main protease NSP5 prevents autophagic degradation of viral envelope
    Article Snippet: The rabbit anti-TOM1 (A9273) pAb, anti-ATG5 (A11427) pAb, anti-BECN1 (A7353) pAb, and anti-MAP1LC3B/LC3B (A21800) pAb, rabbit anti-pro-CASP3/caspase 3 (A19654), and anti-CASP3/caspase 3 (A19664) mAb were purchased from ABclonal Biotechnology.



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    Effects of OST on cardiac autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, <t>Beclin-1,</t> p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.
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    Effects of OST on cardiac autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, <t>Beclin-1,</t> p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.
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    Effects of OST on cardiac autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, <t>Beclin-1,</t> p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.
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    Image Search Results


    Effects of OST on cardiac autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway

    doi: 10.1016/j.jtcme.2025.06.001

    Figure Lengend Snippet: Effects of OST on cardiac autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.

    Article Snippet: The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase-3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA).

    Techniques: Transmission Assay, Electron Microscopy, Western Blot

    Effects of OST on autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice after SB203580 administration. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-mTOR and mTOR. (H) Quantitative analysis of p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). # P < 0.05, ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group; ▲ P < 0.05, ▲▲ P < 0.01 vs the TRZ + OST H group.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway

    doi: 10.1016/j.jtcme.2025.06.001

    Figure Lengend Snippet: Effects of OST on autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice after SB203580 administration. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-mTOR and mTOR. (H) Quantitative analysis of p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). # P < 0.05, ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group; ▲ P < 0.05, ▲▲ P < 0.01 vs the TRZ + OST H group.

    Article Snippet: The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase-3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA).

    Techniques: Transmission Assay, Electron Microscopy, Western Blot